Association Analysis of Solvent Retention Capacity in Soft Wheat

Abstract

Solvent retention capacity(SRC) is an important index for identification and evaluation of soft wheat varieties. This study aimed at identifying SRC associated markers for marker-assisted selection. One hundred and seventy-six wheat varieties(lines) in different hardness types were screened with 236 pairs of SSR primers and their SRC values of lactic acid,water,sucrose,and sodium carbonate were evaluated in three growing seasons in Lixiahe,Jiangsu Province. The association analysis was carried out using the mixed-linear model(MLM). A total of 1340 fragments were amplified on the 236 SSR loci with an average of 5.5 alleles per locus. The average polymorphism information content was 0.4663. Twenty-eight loci were identified to be associated with lactic acid SRC(13),water SRC(7),sucrose SRC(6),and sodium carbonate SRC(2) at the significant level of P 0.005,and a single locus explained 3.19%–21.84% of phenotypic variation. Marker gwm642 associated with WSRC was detected in three years. Some favorable alleles associated with SRCs were found,such as gwm642-A186,gwm642-A188,and gwm337-A178 for reducing water SRC,gwm337-A178 and gwm337-A186 for reducing sucrose SRC,and cfa2257-A129 for reducing sodium carbonate SRC. These results are informative for marker-assisted selection on SRC properties in wheat.