Association analysis of eNOS gene polymorphisms and coronary artery disease

Abstract

Objective To investigate the relationship between the 3 polymorphisms (-786T/C,4a4b,894G/T) in endothelial nitric oxide synthase (eNOS) gene and coronary artery disease (CAD).Methods 148 patients with CAD and 115 healthy unrelated individuals in a Chinese Han population were involved.The genotype and allele frequencies of each polymorphisms of the eNOS gene in these patients and normal controls were examined with polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) or PCR methods.Genotypes and allele frequencies were analyzed by HaploView 4.0 and SPSS 13.0 software.Results There were significant differences in both allele and genotype frequencies of -786T/C and 4a4b between the study and control group.The frequencies of CC genotype of the -786T/C and 4a/4a genotype of the 4a4b in eNOS gene were significantly higher in CAD than that in controls (P<0.05).Between patients with CAD and controls，there were no significant differences in the frequencies of the genotypes and alleles of the 894G/T in eNOS gene.Conclusions The -786T/C and 4a4b polymorphisms of eNOS gene may be associated with CAD.The individuals with C allele of -786T/C and 4a allele of 4a4b are susceptible to CAD.Between CAD and normal controls，there is no significant association in the and 894G/T polymorphism in eNOS gene.