Association analysis between polymorphism of dopamine transporter variable number tandem repeat and breast cancer with abnormal Hilit

Abstract

To explore the association between polymorphism of dopamine 1 transporter variable number tandem repeat (DAT1 VNTR) and breast cancer with abnormal Hilit in Chinese Han population from Xinjiang. The breast caner patients were divided into four body fluids according to Uighur medicine theory. And polymerase chain reaction and VNTR polymorphism technique were employed to detect genotypic and allelic frequencies of a 40 bp VNTR polymorphism situated in 3' untranslated region of DAT1 gene in 144 breast cancer patients with abnormal Hilit and 104 normal control subjects in Han population of Xinjiang province. In our sample, the repeat numbers of 40 bp were 7 and 9 - 11 (PCR product length of 360 bp and 440 bp to 520 bp) and 10-repeat allele (480 bp) detected was the dominant allele of DAT1 gene polymorphisms with an allelic frequency of 90.9%; Seven kinds of genotype were detected and genotype 10/10 was the most common genotype with a genotypic frequency of 83.1%. The frequency of 10-repeats allele and 10/10 genotype was significantly higher in breast caner patients with abnormal balgham Hilit than in controls (OR = 0.127, 95%CI 0.016 - 0.988, P = 0.026; OR = 0.134, 95%CI 0.018 - 1.016, P = 0.020) and breast caner patients with abnormal Sapra Hilit (OR = 0.132, 95%CI 0.016 - 1.075, P = 0.049; OR = 0.132, 95%CI 0.017 - 1.042, P = 0.033). The 10-repeats allele and 10/10 genotype of 40 bp VNTR polymorphism of DAT1 may increase the risk of breast cancer with Uighur medicine abnormal Hilit in Chinese Han population from Xinjiang province and it is not correlated with the susceptibility to breast cancer with Uighur medicine abnormal Sapra Hilit.