Association analysis between Lipoxygenase activity and SSR markers in wheat grains

W Y Zheng,D N Yao,J Li,Y M Li,L Zhao,Z H Zhu

doi:10.1007/s42976-021-00168-8

Abstract

Lipoxygenase (LOX) activity is closely related to wheat processing and storage quality. In the present research, ten wheat cultivars were used to compare the effects of genotype, location, year, and their interactions on the LOX activity. Furthermore, 123 wheat cultivars were evaluated for LOX activity with 192 simple sequence repeat (SSR) markers and to identify elite alleles related to LOX activity. The results indicated that LOX activity was highly affected by genotype (variety) than that by the location. A total of 22 SSR molecular marker loci with a significant or very significant correlation with LOX activity were identified on performing association analysis. In 3 years, only one molecular marker locus associated with LOX activity was detected (WMC488); in 2 years, seven molecular marker loci were detected, while in only 1 year, the other 14 molecular marker loci were detected. A total of 7 and 6 marker loci significantly related to LOX activity accounting for 31.2% and 27.2%, respectively, were located in homologous groups 4 and 5, and group 7. This research provided the theoretical basis and the markers for molecular-assisted wheat breeding that facilitate the breeding process in the processing and storage quality of grains.

Highlights

Lipoxygenases (EC 1.13.11.12., LOX) are isoenzymes that contain non-heme iron and are widely found in many plants
While the location has the least effect, and the effect of the interaction of location, variety, and the year is more important than that of variety and year. It indicated that the difference of varieties had the greatest effect on the LOX activity of wheat, which is consistent with the study results of Borrelli et al (2003)
In results of this study demonstrates that genotype controls the LOX activity, which was determined by multiyear and multipoint experiments; this was consistent with previous studies (Borrelli et al 2008; Geng et al 2011a, b, c)

Summary

Introduction

Lipoxygenases (EC 1.13.11.12., LOX) are isoenzymes that contain non-heme iron and are widely found in many plants. They catalyze the oxygenation of polyunsaturated fatty acids with Cis, Cis-1, and 4-pentadiene structure to produce polyunsaturated hydroperoxides with conjugated double bonds (Guo et al 2017; Boyington et al 1993). Somers et al (2003, 2004) and Geng et al (2011a) developed and indicated several SSR markers interlinked with LOX active QTL on chromosomes 4B and 1A, respectively. Pshenichnikova et al (2008) believed that an AFLP molecular marker Xbcd1262, located in 4BS, was closely linked to LOX activity. Two complementary dominant markers LOX16 and LOX18 were used on the developed 4B to detect the LOX activity

Methods

Results

Conclusion