Abstract
Several studies report that the OPG is an important candidate gene in the pathogenesis of osteoporosis. This study aimed to detect the potential association of OPG gene polymorphisms with osteoporosis in postmenopausal women. We recruited 928 subjects containing 463 with primary postmenopausal osteoporosis and 465 healthy volunteers as controls. The BMD of neck hip, lumbar spine (L2–4), and total hip were assessed by dual-energy X-ray absorptiometry (DEXA). Through the created restriction site-polymerase chain reaction (CRS-PCR), PCR-restriction fragment length polymorphism (PCR-RFLP), and DNA sequencing methods, the g.18873C>T and g.27522G>A have been investigated. As for g.18873C>T, our data indicated that subjects with CC genotype have significantly higher BMD value than those of CT and TT genotypes (all P values < 0.05). As for g.27522G>A, the BMD values of subjects with GG genotype were significantly higher than those of GA and AA genotypes (all P values < 0.05). Our findings suggest that the OPG g.18873C>T and g.27522G>A genetic polymorphisms are associated with the decreased risk for osteoporosis in Chinese postmenopausal women.
Highlights
Osteoporosis is a multifactorial disease in the postmenopausal women, which is characterized by low bone mineral density (BMD) and deteriorated microarchitecture of bone with the increased susceptibility to fracture [1–9]
The polymerase chain reaction (PCR) products of g.18873C>T were digested with AciI restriction enzymes and divided into three genotypes, CC (196 bp and 20 bp), CT
Evidence from the published reports approved that several osteoprotegerin gene (OPG) genetic polymorphisms have been potentially associated with BMD and osteoporosis [6, 7, 9, 10, 16, 18, 22, 25–32]
Summary
Osteoporosis is a multifactorial disease in the postmenopausal women, which is characterized by low bone mineral density (BMD) and deteriorated microarchitecture of bone with the increased susceptibility to fracture [1–9]. It is well accepted that the genetic factors play key roles in the etiology of osteoporosis [11–16]. Some genetic polymorphisms (such as A163G, T245G, T950C, G1181C, g.18861A>G, and g.27406C>T) in OPG gene have been reported to play genetic influence on BMD and osteoporosis [6, 7, 9, 10, 16, 18, 22, 25–32]. There are no similar related studies which reported the relationship of OPG g.18873C>T and g.27522G>A genetic polymorphisms with BMD and osteoporosis. This study aims to detect these two OPG genetic polymorphisms and to assess their potential association with BMD and osteoporosis in postmenopausal women
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