Association analysis between CTLA-4 polymorphisms and bipolar disorder

Abstract

Event Abstract Back to Event Association analysis between CTLA-4 polymorphisms and bipolar disorder Alireza Rafiei1*, Zahra Hosseini-khah1, Hamzeh Hosseini1 and Mariam Vesali1 1 Mazandaran University of Medical Sciences, Immunology, Molecular and Cell Biology Research Center, Iran Background: The immunogenetics of bipolar affective disorder (BPD) comprises a complex area of study with growing importance. In BPD, massive immune activation might be due to immune dysregulation. The current study investigated whether is a contribution of CTLA-4 SNPs in a Caucasian population with bipolar affective disorder (BPD). Methods: This case-control association study assessed the association of four common CTLA-4 SNPs (-318 C/T, -1661 A/G and -1722 C/T in the promoter region and +49A/G in exon 1) with BPD subjects. Genotyping was performed in 312 BPD patients and 308 healthy controls by polymerase chain reaction-fragment length polymorphism or amplification refractory mutation system analysis. Results: The allelic and genotype frequencies of only -1661 A/G SNP were more prominence in BPD patients than that controls (0.0003 and 0.04, respectively). Multivariate logistic regression analysis was demonstrated that G allele carriage genotypes had significantly increased risk of BPD up to 1.9 fold (95% CI; 1.3- 2.7, p= 0.0008). Gender stratification was revealed CTLA-4 -1661 A/G and +49 A/G SNPs are associated with increased susceptibility to BPD only in female patients. In addition, female patients who carried -1661G allele, experienced more depressive episodes than the males patients. Conclusion: Our results suggested that CTLA-4 gene variants might be associated to increased risk of BPD, particularly, in a gender-specific manner.