Association Analysis about HLA-DRB1, -DQB1 Polymorphism and Auto-Antibodies Against α1-Adrenergic Receptors in Chinese Patients with Essential Hypertension

Abstract

The auto-antibodies against α1-adrenergic receptors (α1-AAs) with agonist activity likes norepinephrine have been discovered in patients with essential hypertension but the mechanism of α1-AA production remains unclear. We supposed the α1-AAs be correlated to the HLA-DQB1 and DRB1 alleles. Three hundred ninety-six patients with essential hypertension (EH) and 224 normotensives were enrolled, and DNA typing was detected by protein coupled receptor (PCR) amplification with sequence-specific primers. Analysis was performed by α2 and logistic regression. There were the significant associations of the haplotype HLA-DQB1**0301-DRB1**04 with the prevalence of α1-AAs in hypertensive patients and it obviously added to the risk for the α1-AA production (adjusted P == 0.019, OR 4.037, 95%% CI 1.259–12.947). In normotensives, the haplotype HLA-DQB1**05-DRB1**04 provided a strong predisposition in α 1-AAs production (adjusted P == 0.024, OR 3.922, 95%% CI 1.200–12.817). These results suggest the HLA-DRB1**04 might be the primary risk alleles associated with α1-AA production on the haplotypes HLA-DQB1**0301-DRB1**04 and HLA‐‐DQB1**05-DRB1**04 and increased the risk for α 1-AA production.