Association among B lymphocyte subset and rheumatoid arthritis in a Chinese population

Haiyan You,Cui Ma,Di Chen,Mengwei Cheng,Wenjuan Zheng,Yu Jiang,Yu Tang

doi:10.1186/s13018-021-02883-8

Abstract

Background and aimAutoantibody production are the main risk factors for inflammation of rheumatoid arthritis (RA). This study aimed to investigate differences in B lymphocyte subsets (native B, memory B, and plasmablasts) and several cytokines in RA patients and their correlation with the clinical parameters.MethodsIn total, 81 RA patients (active RA and inactive RA) and 40 healthy subjects were recruited between September 2018 and October 2020. The distribution of B lymphocyte subsets in peripheral blood samples was measured via flow cytometry and the plasma cytokines were detected by enzyme linked immunosorbent assay. The receiver operating characteristic curve (ROC) was used to evaluate the value of each index for RA diagnosis and activity prediction.ResultsThe percentages of native B and memory B cells in RA patients did not differ significantly from the percentages of those in healthy controls. However, the percentage of plasmablasts in active RA patients was significantly higher compared with healthy subjects and inactive RA patients. The percentage of plasmablasts was significantly related to C reaction protein. ROC curve analysis showed that when the best cutoff value of plasmablasts/B cell was 1.08%, the area under the curve (AUC) for diagnosing RA was 0.831 (95% CI 0.748 ~ 0.915), the specificity was 91.4%, and the sensitivity was 67.5%. The AUC predicted by the combination of plasmablast and anti-CCP for active RA patients was 0.760, which was higher than that of plasmablast and anti-CCP.ConclusionIn conclusion, the percentage of plasmablast varies among RA patients in different stages. The percentage of plasmablasts can be used as an early diagnosis marker for RA.

Highlights

Rheumatoid arthritis (RA) is a common inflammatory arthritis [1], with a prevalence of 0.5–1.0% [2]
The positive rates of rheumatoid factor (RF) and CCP antibodies in rheumatoid arthritis (RA) patients were 80.2% and 56.8%, respectively
B lymphocyte subsets, and RA disease The study investigated the distribution of B lymphocyte subsets on CD19 + B cells

Summary

Introduction

Rheumatoid arthritis (RA) is a common inflammatory arthritis [1], with a prevalence of 0.5–1.0% [2]. A large number of cytokines, such as tumor necrosis factor alpha (TNFα) and interleukins (IL)-1, 6, and 15, were found in the joint fluid and blood of patients with active diseases. B cells are activated by activated T helper cells to become plasma cells, which can secrete large amounts of immunoglobulins, including rheumatoid factor (RF) and other antibodies, further causing inflammation and destruction of joints. Human B lymphocytes in peripheral blood can be divided into three subgroups representing different stages of differentiation: native B cells, memory B cells, and plasmablasts. There were few studies underlying their association between B cell subsets and RA. This study aimed to investigate differences in B lymphocyte subsets (native B, memory B, and plasmablasts) and several cytokines in RA patients and their correlation with the clinical parameters

Objectives

Methods

Results

Conclusion