Abstract
It is suggested that embryo hatching and subsequent implantation may be impaired in some patients with embryos with a thicker than normal zona pellucida (Cohen et al., 1992). Secondary zona hardening is also thought to occur during suboptimum in-vitro culture conditions (De FelicisSiracusa, 1982) or after cryopreservation (Carroll et al., 1990). Assisted hatching may enhance embryo implantation by mechanically facilitating the hatching process from the zona pellucida and permitting timely trophoblast-endometrial contact.
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