Abstract

• Bean seedlings (Phaseolus vulgaris cv. Pinto) were grown in the greenhouse at a light intensity of 400µmolm-2 s-1 . When the primary leaf was fully expanded, plants were divided into four groups and subjected to one of the following treatments: light intensity of 400µmolm-2 s-1 and filtered air (control); light intensity of 400µmolm-2 s-1 and ozone (O3 ) (150nll-1 for 5h) (ozonated); light intensity of 1000µmolm-2 s-1 for 5h and filtered air (HL); and light intensity of 1000µmol m-2 s-1 and O3 (150nll-1 ) for 5h (HL+O3 ). • At the end of the treatments (HL and/or O3 ) a strong decrease in CO2 assimilation rate as well a decrease in stomatal conductance were observed, while no changes in intercellular CO2 concentration were recorded. In addition the Fv :Fm ratio (maximal quantum yield for PSII photochemistry) decreased in the stressed leaves (HL and/or O3 ), indicating photoinhibition, and they showed a corresponding increase in minimal fluorescence (F0 ), indicating a higher number of deactivating photosystem II (PSII) centres. • The maximum catalytic activity of the Benson-Calvin cycle enzymes, fructose-1,6-bisphosphate phosphatase (FBPase) and Rubisco, decreased following HL+O3 stress but activation was enhanced. A linear relation was found between activation state of NADP-malate dehydrogenase (MDH) and the flux of electrons through PSII and in HL+O3 -treated plants NADP-MDH activity decreased at high irradiance levels, indicating a limitation in linear electron flux.

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