Assignment of the porcine FUS1 gene to SSC13q21→q22 by somatic cell and radiation hybrid panel mapping

Abstract

FUS1 is a candidate tumor suppressor gene organized in a 370 kb region which contains a set of 19 genes. The human FUS1 genomic sequence spans 3.3 kb containing 3 exons (NM_007275; Lerman and Minna, 2000). Overexpression of the FUS1 gene leads to G1 arrest and growth inhibition of lung cancer cells (Kondo et al., 2001). The human and the murine FUS1 genes were assigned to chromosome HSA3p21.3 and MMU9F1, respectively. The sequence characterization of a porcine PAC clone revealed the identical gene cluster for pigs and human with respect to genes FUS1–HYAL2–HYAL1– FUS2–HYAL3. The porcine HYAL3 gene has recently been mapped to SSC13q21→q22 by fluorescence in situ hybridization (FISH) and radiation hybrid panel analysis while the somatic cell hybrid panel analysis resulted in the localization of the gene to SSC13q42→ (1/2q46) (Gatphayak et al., 2003). Considering the gene order in the cluster, the latter assignment is in accordance with the mapping of the porcine HYAL1 gene – which was provisionally called LUCA1 (for LUng CAncer) – to SSC13q23→ (1/2)q41 by Lahbib-Mansais et al. (1999) also using the somatic cell hybrid panel. Here we report the mapping of the porcine FUS1 gene by somatic cell and radiation hybrid panel and provide enhanced knowledge about the localization of the gene cluster. Methods and materials