Abstract
The direct mapping method combined with fluorescence in situ suppression hybridization and replicated prometaphase R-bands, which is based on cell synchronization with excess thymidine followed by bromodeoxyuridine release, was described elsewhere. The probe used in the present study was a plasmid clone of a 12.5-kb genomic fragment, containing sequences completely identical with those of the coding region in the human PAF-1 cDNA (results not shown). This was isolated from a human placental genomic library constructed in [lambda]EMBL3 SP6/T7 (CLONTECH) by plaque hybridization, using the human PAF-1 cDNA as a probe. Of the 100 R-banded prometaphase preparations examined, 31% exhibited two identical greenish-yellow spots located symmetrically on each chromatid in both homologs of chromosome 8q21.1. Nine percent had twin spots on one homolog of only one homolog, and 22% had a single spot on only one chromatid. The others had no detectable signals. No other chromosomal site exhibited specific signals. Almost all signals were located on the middle part of 8q21.1. Thus, PXMP3 responsible for ZS of complementation group F was mapped to chromosome 8q21.1.
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