Abstract
Six previously unassigned genes have been assigned to the BTA13 by means of somatic cell hybrid analysis. Polymerase chain reaction primer design for the amplification of AHCY, PLTP, PPGB and PCK1 was based on nucleic acids sequence information of homologous genes on HSA20. Primers for PDYN and ASIP were designed from porcine and bovine sequence information, respectively. Homology was established by sequence analysis. These assignments support the previous finding of a conserved syntenic relationship between HSA20 and BTA13 and contribute to the understanding of the chromosomal evolution of BTA13. This is significant since the prion protein gene, thought to play a key rule in the development and course of bovine spongiforme encephalopathy is also located on BTA13.
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