Abstract
AB Aquatic Biology Contact the journal Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsTheme Sections AB 26:69-73 (2017) - DOI: https://doi.org/10.3354/ab00675 Assignment of parentage by microsatellite analysis in the endangered Brachymystax lenok tsinlingensis (Salmonidae) Jian Shao1,2,3, Wei Luo4, Qiwei Wei1,2,*, Feng Wang5, Wei Guo2, Huan Ye2, Zhipeng Chu2, Jinming Wu2, Shuhuan Zhang2 1College of Fisheries, Huazhong Agricultural University, Wuhan 430070, PR China 2Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China 3College of Animal Science, Guizhou University, GuiYang 550025, PR China 4South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China 5Yellow River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Xi’an 710086, PR China *Corresponding author: weiqw@yfi.ac.cn ABSTRACT: We evaluated the use of microsatellite markers to identify parentage in Brachymystax lenok tsinlingensis Li, 1966, an endangered salmonid. Computer simulations showed that when no parent information was available, or when information was only available from 1 parent, then exclusion probabilities ranged from 23.6 to 45.1% and from 25.9 to 52.4%, respectively; combined exclusion probabilities for the 8 loci investigated were 97.7 and 98.4%, respectively. However, a breeding experiment with known parental and filial information resulted in 91.28% of progeny being exclusively assigned to their correct parent pair. Our data demonstrate that microsatellite-based parentage assignment is a reliable means with which to obtain information pertaining to genealogical relationships and could therefore benefit genetic conservation of this species. KEY WORDS: Brachymystax lenok tsinlingensis · Microsatellites · Parentage assignment · Inbreeding depression Full text in pdf format PreviousNextCite this article as: Shao J, Luo W, Wei Q, Wang F and others (2017) Assignment of parentage by microsatellite analysis in the endangered Brachymystax lenok tsinlingensis (Salmonidae). Aquat Biol 26:69-73. https://doi.org/10.3354/ab00675 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in AB Vol. 26. Online publication date: June 19, 2017 Print ISSN: 1864-7782; Online ISSN: 1864-7790 Copyright © 2017 Inter-Research.
Highlights
The genus Brachymystax includes 4 species: B. lenok, B. savinovi, B. tumensis, and B. tsinlingensis (B. lenok tsinlingensis Li, 1966), which are landlocked coldwater salmonid fishes
The use of a microsatellite- verification by PCR-PAGE, these loci were found to based parentage assignment system would allow be highly polymorphic and were steadily amplified progeny from different families to be communally with clear bands from DNA obtained from B. lenok stocked and retrospectively assigned to family of ori- tsinlingensis
Comparing mating design records and parentage assignment data determined by the 8 microsatellite markers, 96.5% and 93.6% of individuals were exclusively assigned to their true dam and sire, respectively
Summary
The genus Brachymystax includes 4 species: B. lenok, B. savinovi, B. tumensis, and B. tsinlingensis (B. lenok tsinlingensis Li, 1966), which are landlocked coldwater salmonid fishes. In order to maintain genetic diversity, optimal survival, and growth traits, the most appropriate practice is to isolate and raise the progeny of different family groups in separate tanks until they are large enough to be tagged physically. This approach takes up too much space and is labor intensive. The use of a microsatellite- verification by PCR-PAGE, these loci were found to based parentage assignment system would allow be highly polymorphic and were steadily amplified progeny from different families to be communally with clear bands from DNA obtained from B. lenok stocked and retrospectively assigned to family of ori- tsinlingensis. We used known parentprogeny relationships to test the power of selected loci
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