Assignment of CRSP9, ETF1 and TMEM59 genes to porcine chromosomes

Abstract

Several porcine cDNA clones were isolated from Chinese Tongcheng pig 55-day fetal longissimus dorsi muscle cDNA library and sequenced (Zhu et al., 2005). After BLAST search in the GenBank non-redundant (nr) sequence database (http://www.ncbi.nlm.nih.gov/BLAST/Blast.cgi), we found and identifi ed these three porcine orthologous genes. Primers to amplify across the putative intron regions were designed based on the pig cDNA sequences after alignment of the pig and human sequences. PCR products were confi rmed by sequencing and then pig-specifi c primers were designed in intron regions. Chromosome location was identifi ed by PCR analysis of the porcine whole genome hybrid panel. Each PCR was performed in duplicate according to the protocol described by Wang et al. (2005). The PCR profi le included 3 min at 94 ° C; 35 cycles of 20 s at 94 ° C, 20 s at 62 ° C, and 20 s at 72 ° C, and a fi nal 5 min extension at 72 ° C. Data analysis was performed through the IMpRH web site (http://www.toulouse.inra.fr/lgc/pig/RH/IMpRH.htm).