Assidik sıvı hücre sayımında otomatik yöntem yeterince güvenilir bir seçenek midir

Abstract

Background and aims: Ascitic fluid cell count is used routinely in analysis of ascitic fluid. In this study, two methods used in ascitic fluid cell count were compared (automatic method in which a hemogram device is used and standard manual hemocytometer count method in which a Thoma lam is used). Materials and methods: Twenty-six patients (20 men, six women, age range: 19-63 years) admitted to our clinic with ascites were included in the study and ascites fluid obtained from each patient, following which cell counts were performed by both methods. Results: A total of 44 ascitic fluid samples were examined. The upper limit of normal total White Blood Cell (WBC) count (>500 cell/mm3) was exceeded in 23 samples analyzed by the automatic method and 14 samples by the Thoma lam method. Nine out of 23 patients in whom cut-off levels were exceeded by automatic method had normal results with the Thoma lam method. There was no culture-positive result in any of these nine patients. Conclusions: Overdiagnosis may be obtained by use of the automatic method in evaluating ascitis fluid cell counts and the results of this study indicate that further investigation of this subject should be undertaken.