Assessment the Role of Bisphenol A on Chemotherapeutic Efficacy of Cisplatin against Hepatocellular Carcinoma in Male Rats

Abstract

Aim: the objective of this study was to investigate the effect of bisphenol A (BPA) in DENA and CCl4 induced hepatocellular carcinoma male rats. Materials and Methods: DENA was given as a single intraperitoneal injection of freshly prepared (200 mg/kg.bw)for initiation the carcinoma and CCl4 once every week (3ml/kg.bw) for 10 weeks to promote the carcinogenic effect of DENA in male rats. In addition, the study aimed to elucidate the chemo-resistant role of bisphenol A (322 mg/kg.bw) against cisplatin chemotherapeutic effect, as a potent antineoplastic agent in the treatment of hepatocellular carcinoma male rats induced by DENA and CCl4. Results: obtained result showed that the group of rats which was orally administrated by BPA , as well as hepatocellular carcinoma treated rats group significantly have increased relative liver/body weight ratio, serum ALT, AST, ALP, serum total bilirubin, serum total protein and serum AFP and TNF-α, serum TL, TC,TG,LDL,V-LDL levels while a decrease in HDL level .Additionally there was an elevation in hepatic MDA, H2O2 and a significantly depression in SOD, CAT, GST activities and GSH level, as well as an increases in hepatic p53, caspase-3 while a decrease in Bcl-2 level .on the other hand an intraperitoneal injection of cisplatin (1.5mg/kg /bw) repeated two times a week for 3 weeks to the hepatocellular carcinoma induced rats group showed amelioration in the above parameters significantly more than that of hepatocellular carcinoma group treated also with BPA. Conclusion: the consumption of BPA might resist the cisplatin chemotherapy treatment of hepatocellular carcinoma in male rats.