Assessment the cytotoxicity of pomegranate extracts on leukemia cell line

Abstract

The most abundant phytochemicals in pomegranate fruit are high antioxidant properties. Free radicals in excess cause cell and tissue damage, which can lead to asthma, Alzheimer's disease and cancer. This study investigates the antioxidant capacity of phenol extract of pomegranate fruit by scavenging activity against 1,1-diphenyl-2-picrylhydrazine (DPPH). The scavenging activity of the pomegranate fruit extracts was determined at the concentrations 12.5, 25, 50, 100, and 200 µg mL-1. The results for phenol extracts recorded 16.74, 25.12, 40.63, 68.87 and 81.98% respectively compared with ascorbic acid. The immunomodulatory effect of phenol extract on HL60 cell line exhibited a decrease in the levels of tumor necrosis factor alpha (TNF-α). Anti-inflammatory was stimulated when Leukemia cell line treated with phenol extract at concentrations 200 and 400 µg ml-1, TNF-α were significantly reduced and the greater reduction at the concentration 400 µg ml-1. The reduction ranged from 299.30 to 129.6 Pg mL-1 when compared with control (untreated) cells. Additionally, interleukin produced by immune system was estimated after treatment of leukemia cells with the extracts which led to a decreased in the amount of interleukin produced to (15.40 and 7.79 pg mL-1) at (200 and 400 µg mL-1) respectively when compared to untreated cells.