Assessment the ability of Trichoderma harzianum Fungi in Bioremediation of some of Heavy Metals in Waste Water

Abstract

The study included the use of Trichoderma harzianum for biodegradation heavy metals in a wastewater treatment plant and their ability to decompose these minerals through a series of tests. The first test involved the use of two Trichoderma harzianum isolates for biodegradation through a filtration action. In the second test, the comparison was used in pre and post-treatment in Petri dishes, The result showed a clear green growth of fungi and their use of heavy metals as an energy source. Scanning Electronic Microscope SEM) was used to compare the vegetative growth of fungi before and after treatment on P.D.A media, which showed clear and normal growth with a short growth period of the Petri dish after treatment and compared it to the control of the Petri dish with different magnification powers (6738x, 5988x, 2713x, 2509x)., 6453x, 5708x, 2598x, 2400x). A third test used a Atomic Absorption technique to quantify heavy metals for the site 2 after 28 days of bioremediation. The results showed the highest removal rate of Cd was 98.63% and Mn was94.35% for (T.h1) and the lowest biodegradation of Zn and Fe (39.77% and 47.43%,) respectively. The nutrient had the highest removal rate of PO4 was 69.87% and the lowest percentage of NO3 was 8.91%.The results also showed the highest removal rate for (T.h2) Mn and Cd with rates of 86.41% and 81.73%, the lowest percentage of decomposition of Zn was 52.27%, and the highest percentage of decomposition of PO4 was 70.74% Lowest removal rate of NO3 was 36.49%. A Atomic Absorption technique was used to quantify heavy metals after 28 days of biological treatment for the site1. The results showed the highest biodegradation percentage (T.h1) for manganese and Pb 83.27% and 84.50%, respectively, and the lowest biodegradation percentage was Zn and Fe (33.67%, 38.09%,) respectively, and the nutrient removal rate was the highest, NO3 was 85.27 %, The lowest rate is 27.74% of SO4. The results showed the highest removal rate (T.h2) for the elemental fungus Mn (87.32%) and Pb (84.01%) and the lowest percentage for Fe was (36.90%).The results of the experiment showed that the fungi largest biomass are the most efficient in the bioremediation process and the removal of pollutants from wastewater.