Abstract
BackgroundSince yeast Saccharomyces cerevisiae and its components are being used for the prevention and treatment of enteric diseases in different species, they may also be useful for preventing Johne’s disease, a chronic inflammatory bowel disease of ruminants caused by Mycobacterium avium spp. paratuberculosis (MAP). This study aimed to identify potential yeast derivatives that may be used to help prevent MAP infection. The adherence of mCherry-labeled MAP to bovine mammary epithelial cell line (MAC-T cells) and bovine primary epithelial cells (BECs) co-cultured with yeast cell wall components (CWCs) from four different yeast strains (A, B, C and D) and two forms of dead yeast from strain A was investigated.ResultsThe CWCs from all four yeast strains and the other two forms of dead yeast from strain A reduced MAP adhesion to MAC-T cells and BECs in a concentration-dependent manner after 6-h of exposure, with the dead yeast having the greatest effect.ConclusionsThe following in vitro binding studies suggest that dead yeast and its’ CWCs may be useful for reducing risk of MAP infection.
Highlights
Since yeast Saccharomyces cerevisiae and its components are being used for the prevention and treatment of enteric diseases in different species, they may be useful for preventing Johne’s disease, a chronic inflammatory bowel disease of ruminants caused by Mycobacterium avium spp. paratuberculosis (MAP)
Effect of yeast derivatives on the viability of MAC-T cells and Bovine intestinal epithelial cell (BEC) MAC-T cell viability was significantly reduced by Cell wall component (CWC) from all strains as indicated by linear contrasts (p < 0.01, Fig. 3a-d)
BEC viability was significantly increased by inactive yeast from strain A as indicated by quadratic contrasts, and cytotoxicity was not observed for either form of dead yeast from strain A (p < 0.01, Fig. 4e-f )
Summary
Since yeast Saccharomyces cerevisiae and its components are being used for the prevention and treatment of enteric diseases in different species, they may be useful for preventing Johne’s disease, a chronic inflammatory bowel disease of ruminants caused by Mycobacterium avium spp. paratuberculosis (MAP). This study aimed to identify potential yeast derivatives that may be used to help prevent MAP infection. Dairy producers have been using commercially available yeast probiotics and their components as feed supplements for nearly two decades based on claims that these products will improve animal production, promote health, and reduce the need for antibiotic use. Studies demonstrate that supplementing the ruminant diet with specific strains of S. cerevisiae improves feed intake [1, 2], weight gain [3], and fiber digestion [4, 5].
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