Assessment of wound healing activity and safety profile of Amaranthus spinosus extract using incision wound healing model

Abstract

The objective of the study was to determine efficacy of Amaranthus spinosus extract against inflammation and wound along with assessment of safety profile of drug upon topical application. The collected whole plant was extracted with ethanol (65 %). In vitro efficacy of extract was performed on HaCaT and MEF cells. Clonogenic and scratch assays were carried out to determine the wound healing activity of the extract. Acute oral and dermal toxicity studies were conducted in accordance with OECD recommendations in order to assess the drug's profile for safety. Biological efficacy of A. spinosus was determined using incision wound healing model and measuring the tensile strength of a healing skin. Results showed that A. spinosus extract significant reduced inflammation and wound area. The percentage viability of cells were 96.20 and 96.23 %, respectively for HaCaT and MEF cells after 48 h of treatment; however, surviving factor were 0.71 ad 0.79, respectively. The tensile strength was 574.5 g after treatment with drug as compared to the control group. It can be concluded based on findings that A. spinosus may be used as an alternative medicine in the healing of wound.