Abstract
Upon contact with water, two types of rainbow trout ova can be distinguished: ova causing turbid water and ova that do not cause such an effect. The objective of this study was to evaluate the quality of eggs in relation to their ability to cause water turbidity. The biochemical characteristics of ovarian fluid were also determined. When water was used as the fertilization medium, a clear difference in fertilization rate was observed between both types of ova. The percentage of eyed embryos for eggs causing turbid water (15.7±3.0%) was significantly lower than eggs causing non-turbid water (67.2±9.6%). No differences were found when salt solutions were used as the fertilization medium. Protein and lipid concentrations of ovarian fluid collected from eggs causing turbid water were significantly higher than in samples collected from eggs that did not cause turbid water. Our results indicate that the turbidity of water was caused by the coagulation of water with egg contents. In our opinion, performing turbidity tests might be critical for successful fertilization when water is used as the fertilization medium. In such instances, ova causing turbid water must be discarded. The test for turbidity should provide valuable data concerning initial quality of eggs.
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