ASSESSMENT OF VIRULENCE FACTORS OF CANDIDIAL SPECIES AND ITS BIOTYPING IN ORAL POTENTIALLY MALIGNANT DISORDERS

Abstract

The aim of the study was to evaluate the virulence factors of candidal species and its biotypes associated with Oral Potentially Malignant Disorders. Materials and methods included that the present study comprised of red and white lesions (40) and normal healthy control group (5) individuals. Oral swabs were taken from representative area of the lesion to be used for both cytological, microbiological and biochemical tests. Samples were inoculated for fungal growth in Sabouraud Dextrose Agar and culture-positive samples had undergone for the germ tube test, chlymadospore formation and chrome agar test. The virulence factors were conrmed by Lipolytic, proteolytic, protinease and phospholipase tests. Anova test, Kruskall Wallis test and Post Hoc test were the statistical analysis used. The result showed that in this study group no candidial growth was found in control group, study group showed positive germ tube test formation, chlymadospore formation and chrom agar test. All positive isolates produced the four virulence factors with higher levels in candida albican isolates. The hemolytic activity in leukoplakia was found to be more virulent followed by Protienase activity amongst the group and within the study groups. Phospholipase activity amongst the study groups was found more virulent in Oral squamous cell carcinoma. Lipolytic activity was found more aggressive in Denture induced stomatitis amongst all the study group. It can be concluded that in this study there is a close association of Candida species with Oral potentially malignant disorders. The correlation of virulence properties of Candida with potentially malignant disorders has led to its role in its invasive potential.