Assessment of UV-B damage in cyanophage PP

Abstract

AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials AME 58:323-328 (2010) - DOI: https://doi.org/10.3354/ame01386 Assessment of UV-B damage in cyanophage PP Ming-Jun Liao1,2,3, Kai Cheng2, Jiao-Yan Yang2, Yi-Jun Zhao2,*, Zheng-Li Shi1,** 1State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, PR China 2Hubei Key Laboratory of Urban Water Environmental Ecology, Central China Normal University, Wuhan 430079, PR China 3Graduate School of Chinese Academy of Sciences, Beijing 100039, PR China Corresponding authors. Email: *zhaoyj@mail.ccnu.edu.cn; **zlshi@wh.iov.cn ABSTRACT: Cyanophage PP is a short-tailed, icosahedral-shaped, double-stranded DNA virus and can be frequently detected with high abundance and activity in many eutrophic lakes in China. Solar radiation is one of the main factors affecting cyanophage infectivity. In this study, cyanophage PP was treated with different intensities of UV-B radiation, and the accumulation of cyclobutane pyrimidine dimers (CPDs), viral particle destruction and viral attachment ability were analyzed. The viral infectivity decay rate under white and red light was also studied using plaque-forming assays. The results indicate that (1) the level of CPDs induced by UV-B was significantly correlated with radiation intensity, while exposure dose had little effect on the DNA damage level, (2) the level of CPDs was significantly correlated with both the decay rate and the repair rate in cyanophage PP, and (3) UV-B could result in the destruction of viral particles and the decrease in viral attachment. This work suggests that the secondary and tertiary structure of the DNA may influence the formation of CPDs, and that capsid damage caused by UV-B radiation may play an important role in viral decay and survival. KEY WORDS: Cyanophage PP · UV-B · Cyclobutane pyrimidine dimers · CPDs · Capsid damage Full text in pdf format PreviousCite this article as: Liao MJ, Cheng K, Yang JY, Zhao YJ, Shi ZL (2010) Assessment of UV-B damage in cyanophage PP. Aquat Microb Ecol 58:323-328. https://doi.org/10.3354/ame01386 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in AME Vol. 58, No. 3. Online publication date: February 11, 2010 Print ISSN: 0948-3055; Online ISSN: 1616-1564 Copyright © 2010 Inter-Research.