Abstract
BackgroundShrimp allergy is a growing problem among the European population. TSLP, IL-25 and IL-33 are involved in the pathophysiology of allergic diseases, including asthma and atopic dermatitis, as they activate the Th2-dependent immune response.MethodsThirty-seven patients (18 male and 19 female) with a positive history of symptoms associated with shrimp consumption were selected. All patients had blood samples taken to assess the concentration of allergen-specific IgE (sIgE) to house dust mites (HDM) and shrimp (Singleplex, quantitative method with cut off value > 0,35 kAU/L) as well as the level of allergen components using the ImmunoCap ISAC method (Microarray test, semi-quantitative with cut off value > 0,3 ISU-E). The concentrations of TSLP, IL-25 and IL-33 in the patients’ blood serum was assessed using the ELISA method (Cusabio). Twenty patients with negative allergy history of allergic disease tests were included in the control group.ResultsAmong the 37 shrimp-allergic patients, ImmunoCap ISAC was identified the presence of sIgE to the available shrimp allergen components in only 14 cases (37.8%). TSLP and IL25 levels were significantly higher in the study group. No statistically significant correlation was found between the concentration of analyzed alarmins and the concentration of sIgE level to shrimp or HDM between the study and control groups. No statistically significant correlation was found between poly-sensitization occurring in patients and levels of TSLP, IL-25 and IL-33 .ConclusionIn shrimp-allergic patients, the concentrations of TSLP and IL-25 were significantly higher than in the control group (1.33 vs. 0.49 and 157 vs. 39.36, respectively). There was no correlation between the concentrations of TSLP, IL-25 and IL-33 and the concentration of sIgE in the patients or the number of allergen components that the patients were sensitized to.Trial registration: Bioethics Committee 147/2015, 11.03.2015.
Highlights
Shrimp allergy is a growing problem among the European population
Among the 37 patients with a history of self-declared shrimp-related allergy symptoms post consumption and elevated shrimp-specific Immunoglobulin E (IgE) (ImmunoCap), ImmunoCap ISAC identified the presence of sIgE in at Reported symptoms aŌer shrimp*
Among 37 patients with a history of shrimprelated symptoms and elevated shrimp-specific IgE (ImmunoCap), ImmunoCap ISAC identified the presence of sIgE to available shrimp allergen components in only 14 cases (37.8%)
Summary
Shrimp allergy is a growing problem among the European population. TSLP, IL-25 and IL-33 are involved in the pathophysiology of allergic diseases, including asthma and atopic dermatitis, as they activate the Th2dependent immune response. The role of TSLP is extremely important in the development of allergic inflammation in the airways. An interesting report was published in 2009 by Headley M et al The researchers evaluated the respiratory response of mice to TSLP, administered alone or in combination with ovalbumin (OVA), every other day for two weeks. It was found that the mice treated concomitantly with TSLP and OVA showed significantly more pronounced airway inflammation and eosinophilia compared to mice receiving TSLP alone. These results indicate that allergen exposure and TSLP interaction play the important role of the antigen in the development of inflammation of allergic etiology [4]
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