Abstract
Phenols and flavonoids are secondary metabolites of the plant and play a key role in scavenging free radicals and act as antioxidant. Costus speciosus (Costaceae family) and Catharanthus rosea (Apocynaceae family) are important ornamental and ethno medicinal plant rich in bioactive compounds used for treatment of several types of diseases. The plants are well known for their various pharmacological activities such as anti-diabetic, anticancer, antibacterial, antifungal, antioxidant, anti-hyperglycemic, anti-inflammatory, analgesic, antipyretic, anti-diuretic, antiulcer and anti-diarrheal activities. In the present study the total phenol and flavonoid content of root methanol extract of both plants were determined from five blocks of Balaghat district Madhya Pradesh. Folin-Ciocalteau assay was used to determine total phenol content and aluminium chloride method for flavonoid content using gallic acid and quercetin as standard respectively. The result showed highest concentration of total phenol content in Birsa block for both C. speciosus (95.85±1.56 mg GAE/g) and C. rosea (82.32±0.81 mg GAE/g). The total flavonoid content was found highest in Paraswada block for C. speciosus (97.67±0.53mg/QE) and Baihar block for C. rosea (93.20±0.54mg/QE). The ratio of flavonoid to the phenolic in each extract was also determined to as certain extracts that are rich in flavonoids.
Highlights
Phenols and flavonoids are secondary metabolites of the plant and play a key role in scavenging free radicals and act as antioxidant
Phenol and flavonoid compounds are a class of antioxidant agents which act as free radical terminators and their bioactivities may be related to their abilities to chelate metals, inhibit lipooxygenase and scavenge free radicals
Plant materials rich in antioxidants are used in the food industry because they decrease the oxidative degradation of lipids and maintain the quality and nutritional value of food (Kahkonen et al 1999).The aim of the present study was to determine the total phenol and flavonoid content in methanol root extracts of Costus speciosus and Catharanthus rosea collected from five blocks of Balaghat district, Madhya Pradesh
Summary
Phenols and flavonoids are secondary metabolites of the plant and play a key role in scavenging free radicals and act as antioxidant. Folin-Ciocalteau assay was used to determine total phenol content and aluminium chloride method for flavonoid content using gallic acid and quercetin as standard respectively. The result showed highest concentration of total phenol content in Birsa block for both C. The total flavonoid content was found highest in Paraswada block for C. speciosus (97.67±0.53mg/QE) and Baihar block for C.
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