Abstract
The tobacco-specific nitrosamines (TSNAs) are one of the most important groups of carcinoigens in tobacco leaf, knowing the difference of TSNAs contents in tobacco genotypes should be beneficial for breeding low TSNAs cultivars which could decrease the harmfulness to human health in tobacco products. For this purpose, 8 genotypes (include burley, sun-cured and flue-cured tobacco) were selected as materials, the TSNAs contents of uncured and cured leaves were assessed with LC-MS/MS. The results showed that TSNAs contents were significantly increased after curing in all genotypes. The mean TSNAs contents of leaves before and after curing in burley were the highest among three types of tobacco, and the contents in sun-cured tobacco were the lowest. Coefficient of variation (CV) of TSNAs among genotypes in burley was higher than that in flue-cured and sun-cured tobacco, and the CV in sun-cured tobacco was the lowest. The positive correlation was observed in NNN, NAT and total TSNAs contents between uncured and cured leaves in three types of tobacco. Therefore, the TSNAs contents in uncured leaves could be considered as evaluation index to screen tobacco cultivars with the low TSNAs contents.
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