Assessment of the Two-Glutathione S-Transferase Genotypes GSTM1 and GSTT1 Polymorphism Frequencies in Epithelial Ovarian Cancer in Nasiriya Province

Abstract

The family of glutathione S-transferases (GSTs) is composed of multiple isozymes with significant evidenceof functional polymorphic variation. Glutathione S-transferase GSTM1 and GSTT1 are members of theGST family, is responsible for metabolism of xenobiotics and carcinogens. Allelic variant of GST genepolymorphisms might impair detoxification function and increase the susceptibility to cancer. The nullgenetic polymorphism in the gene encoding GST Class µ, GSTM1 and GSTT1, has been reported to besignificantly elevated in cancer patient. This study aimed to investigate if these polymorphisms are usefulmarkers for predicting ovarian cancer susceptibility. 30 cases of epithelial ovarian cancer and 30 controlswas used to investigate the frequency of GSTT1 and GSTM1 genotypes by using multiplex conventionalPCR protocol. The frequency of GSTM1 null allele was significantly elevated (100%, P = 0.004) comparedwith GSTT1 active allele (13% P = 0.037). GSTT1 null showed no significant effect in prognosis epithelialovarian cancer (17%, P = 0.551). We conclude that the GSTs might affect in both risk to ovarian cancer.