Abstract
Biological therapy in the treatment of immune-mediated conditions has changed their course, the quality of life of patients and the prognosis of diseases. The accumulated by mankind 20 years of experience with the use of genetically engineered drugs has led to a number of questions regarding, among other issues, safety in the long-term administration of biological therapy. Patients suffering from ulcerative colitis revealed changes in cell membranes, reflecting their structural and energy characteristics. Long-term administration of Infliximab leads to the stabilization of energy processes in the erythrocyte membrane and improves homeostatic function of the kidneys.The aim of the studywas to evaluate the effect of long-term use of TNF-α blockers (Infliximab) on the structural and functional characteristics of cell membranes and the functional state of the kidneys in patients with moderate to severe ulcerative colitis.Materials and methods. We examined 103 patients with moderate to severe ulcerative colitis during the period of acute attack and remission, of which 28 patients received basic therapy using the drug Infliximab (IFX) for 10 years, 75 patients received standard basic treatment. The patients of the biological therapy group took the original drug Infliximab – Remicade. The comparison group consisted of 30 healthy volunteers, comparable by sex and age. The analysis of the state of erythrocyte membranes was carried out using a set of physicochemical methods: UV spectroscopy (SF-46m spectrophotometer), high-performance thin-layer reaction paper chromatography, membrane ultrafiltration, erythrocyte NMR spectroscopy on phosphoric (31P) and proton (1H) nuclei. The functional state of the kidneys was evaluated using a dynamic scintigraphic study (with the technemage –Tc-99m).Results.Prolonged use of anticytokine therapy with Infliximab for 10 years in patients with ulcerative colitis, upon reaching deep remission, improves endogenous intoxication, restores the structural and functional characteristics of cell membranes, normalizes cell energy metabolism and does not negatively affect the functional state of the kidneys.
Highlights
Язвенный колит (ЯК) – хроническое иммунное заболевание толстой кишки, сопровождающееся деструктивными изменениями её слизистой оболочки, развитием внекишечных и системных проявлений
The accumulated by mankind 20 years of experience with the use of genetically engineered drugs has led to a number of questions regarding, among other issues, safety in the long-term administration of biological therapy
Patients suffering from ulcerative colitis revealed changes in cell membranes, reflecting their structural and energy characteristics
Summary
Обследовано 103 пациента со среднетяжёлым и тяжёлым течением язвенного колита: Группа пациентов биологической терапии составила 28 человек; пациентов, получающих только стандартное базисное лечение (5-АСК, месалазины, глюкокортикостероидные гормоны, иммунодепрессанты) – 75 чел. Инфузии IFX проводили стандартно, согласно утверждённой инструкции, дозу рассчитывали 5 мг на килограмм веса пациента, с индукционным курсом 0–2–6-я неделя и далее каждые 8 недель, специально подготовленным персоналом в оснащённом для АЦТ кабинете. Среднее количество инфузий – 26 ± 5,4 (min – 8 инфузий – 1 год лечения; max – 45 инфузий – более 8 лет). Соотношение мужчин и женщин – 1 : 1,9, средний возраст – 34,5 ± 1,3 года (18–75 лет). Для оценки функционального состояния почек проводилось динамическое сцинтиграфическое исследование (с технемагом Тс-99m), с определением эффективного почечного плазмотока [10]. Для корреляционной оценки использовали коэффициент ранговой корреляции Спирмена.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Acta Biomedica Scientifica (East Siberian Biomedical Journal)
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
