Assessment of the potential of microbial consortium for the reclamation of mine tailings containing potentially toxic elements

Abstract

Bioremediation using microorganisms is an emerging green technology for the remediation of potentially toxic elements (PTEs) in soils and sediments. However, such technology can differently impact PTEs dynamics (e.g., immobilization and mobilization), ultimately affecting the efficiency of the remediation programs. In this study, we aimed to assess different microbial remediation mechanisms triggered by a microbial consortium to bioremediate Fe-rich mining tailings. The tailings were incubated in a mesocosm system for 35 days with increasing colony-forming units (CFU) of a specific microbial consortium (Azospirillum sp., Pseudomonas sp., Saccharomyces sp., and Rhizobium sp.). At the end of the experiment, we determined the geochemical fractionation of Fe and PTEs in the solid phase to assess the effect of treatments on PTE’s bioavailability. Increasing the CFU resulted in higher Fe (15%) and Mn (37%) reductive dissolution compared to the control. As a result, the Fe and Mn concentrations in water increased by 9-fold. In addition, microbial consortium decreased the contents of Fe and Mn associated with oxides (-59% and −79%, respectively) and increased the more bioavailable solid fractions. The microbial consortium also efficiently decreased PTEs pseudo total contents in the mine tailings (Cr: −85%, Cd: −61%, Pb: −55%, and Cu: −49%). In addition, lower CFUs increased PTEs dissolved in the drainage water, indicating a potential for assisting other remediation strategies. Lower CFU also induced high Cr biomineralization (94%). In conclusion, our work provides novel evidence of a microbial consortium for remediating Fe mine tailings through different strategies (biodissolution and biomineralization). In view of the effects of the microbial consortium over Fe and Mn oxyhydroxide dissolution rates, further research should test it on microbially assisted phytoremediation protocols.