Abstract
AbstractA three-dimensional human tissue model has been developed in our laboratory and used as a substrate for assessing ocular cytotoxicity and irritancy potential. The full-thickness model is a coculture of dermal fibroblasts and epithelial cells (keratinocytes). Metabolically and mitotically active fibroblasts are seeded onto medical-grade nylon mesh, where they attach and secrete collagen and extracellular matrix proteins. Then keratinocytes are seeded onto the top of this submerged stromal tissue, and the tissue grows into a multilayered pithelium. Histologic examination of this cellular coculture system reveals a cellular organization similar to the rabbit/human cornea. The resulting substrate has been used effectively to study the effects of a variety of test compounds (surfactants, powders, creams) at diluted or full-strength concentrations in a time-course assay. The protocol mimics the way in which in vivo animal testing (Draize eye testing) is performed. The treated tissue is assayed for cytot...
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Toxicology: Cutaneous and Ocular Toxicology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
