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Abstract
Jal Brahmi (Bacopa monnieri L.) is an important medicinal herb rendering mental health-promoting activities. In-vitro mass multiplication of elite genotypes of Jal Brahmi may bring off its high industrial demand. In the present study, the comparative in-vitro responses of two diverse genotypes (DBM-4 and DBM-12) were assessed. Direct shoot organogenesis was reported in both genotypes studied in basal MS medium media. However, both genotypes formed calli in the presence of 2, 4-D and IAA. Germplasm DBM-4 observed significantly higher antioxidant potential than that of DBM-12. Further, in both genotypes, methyl jasmonate elicitation significantly improved the antioxidant potential and total phenolic contents of the callus. Hence, DBM-4 has the potential for in-vitro mass multiplication and secondary metabolites production.
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