Assessment of the Genetic Connectivity Between Farmed Populations on a Typical Kelp Farm and Adjacent Spontaneous Populations of Saccharina japonica (Phaeophyceae, Laminariales) in China

Tifeng Shan,Li Su,Shaojun Pang,Xuemei Wang,Qianxi Li

doi:10.3389/fmars.2019.00494

Abstract

The commercially important brown alga Saccharina japonica in China has been believed to be accidentally introduced from Japan in 1920s. Meanwhile, spontaneous populations in the wild are assumed to be derived from the locally farmed populations. Spontaneous populations are often observed in the subtidal zones and on the cultivation infrastructure near farmed populations in the north of China. However, the genetic connectivity between these sympatric spontaneous and farmed populations remains unclear. Here, three commonly farmed cultivars (farmed populations) and three spontaneous populations (two from subtidal zones and one from cultivation rafts) were sampled from a typical kelp farm in Dalian, China, and analyzed with ten polymorphic microsatellite markers. Genetic diversity of farmed populations was found to be higher than that of the subtidal spontaneous populations. Neighbor joining cluster analysis based on genetic distance, Bayesian model-based structure analysis, and discriminant analysis of principal components revealed significant genetic divergence between the farmed populations and the subtidal spontaneous ones. Gene flow out of farmed populations to the subtidal spontaneous populations was revealed to be very limited, but gene flow in the contrary direction was more prominent. The spontaneous sporophytes on the structural rafts contained pedigree from both farmed and subtidal spontaneous populations. Results of this study may help us to understand reciprocal impacts between sympatric spontaneous and farmed populations of S. japonica

Highlights

The important economic kelp species Saccharina japonica
Subtidal spontaneous populations were collected from two rocky locations adjacent to the farmed populations, with the individuals sampled from the west and east regarded as two separate populations, designated as SW
As expected, farmed populations were found to have exerted very limited impact on the genetic composition of the sympatric subtidal spontaneous populations. This was consistent with the results found by Zhang et al (2017), the spontaneous population appeared to be far from the farmed populations investigated in that study

Summary

Introduction

The important economic kelp species Saccharina japonica Druehl, and G.W. Saunders has been assumed to be non-native to China, but unintentionally introduced from the north of Japan in 1920s (Tseng and Zhang, 1952; Tseng, 2001). Saunders has been assumed to be non-native to China, but unintentionally introduced from the north of Japan in 1920s (Tseng and Zhang, 1952; Tseng, 2001) This assumption is supported by the recent genetic diversity analysis of S. japonica populations from Japan and China as founder effect is revealed to be evident in Chinese populations (Shan et al, 2017). Original spontaneous (wild) populations of S. japonica in rocky shores of these regions were thought to be derived from the zoospores escaping from cultivated individuals (Tseng and Zhang, 1952). The southernmost distribution of wild S. japonica populations in China is around 36◦N latitude (Pang et al, 2007)

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Conclusion