Assessment of The Effects Of Crude Metabolic Extracts (Leaf And Twig) of Loranthus Micranthus On Streptozotocin Induced Diabetic Rats

Abstract

The antidiabetic effects of crude methanolic extracts of the leaf and twig of Loranthus micranthus was evaluated in Wister rats. To assess this, data were obtained for the determinant parameters of diabetic complications. Streptozotocin was administered for induction of diabetes; diabetic state was confirmed by persistent hyperglycemia (FBG ≥ 300mg/dl) at 72 hours post induction. Invitro inhibitory activity on α- amylase and α-glucosidase was assayed. Serum insulin, TNF-α, Total cholesterol, HDL, LDL, TG, atherogenic index, liver glycogen and glycated haemoglobin were evaluated. Histology of the pancreas was assessed. Phytochemical analysis revealed the presence of unique compounds in both extracts. In-vitro assay showed inhibitory effects of both extracts on α-amylase and α-glucosidase activity. Hyperglycemia was controlled in both extract-treated groups comparable to glibenclamide. Weight loss after diabetic induction was ameliorated in extracts treated groups; serum insulin level of the extracts-treated and glibenclamide treated-group were higher than the diabetic control group. Serum TNF-α level of extracts-treated and glibenclamide-treated groups were significantly lower than the diabetic control group. Glycated haemoglobin levels of diabetic control group were higher than the extracts treated and glibenclamide group. Dyslipidemia observed in the diabetic control group were ameliorated in all extract-treated groups; atherogenic index of diabetic control group was higher than extracts-and-glibenclamide treated groups. Histopathological assessment showed that the leaf and twig extracts of Loranthus micranthus may possess β cell regenerating activity. Findings from this study suggest that the leaf and twig extracts of Loranthus micranthus ameliorate symptoms and complications of streptozotocin-induced diabetes in rats.