Assessment of the effect of intraarticular injection of autologous adipose-derived mesenchymal stem cells in osteoarthritic dogs using a double blinded force platform analysis.

Jose M Vilar,Miguel Batista,Mónica Rubio,Belén Cuervo,Ramón Cugat,Jose M Carrillo,Angelo Santana,Joaquín Sopena,Manuel Morales

doi:10.1186/1746-6148-10-143

Abstract

BackgroundRegenerative medicine using Mesenchymal Stem Cells (MSC) alone or combined with Plasma Rich in Growth Factors (PRGF) is a rapidly growing area of clinical research and is currently also being used to treat osteoarthritis (OA). Force platform analysis has been consistently used to verify and quantify the efficacy of different therapeutic strategies for the treatment of OA in dogs including MSC associated to PRGF, but never with AD-MSC alone.The aim of this study was to use a force platform to measure the efficacy of intraarticular ADMSC administration for limb function improvement in dogs with severe OA.ResultsTen lame dogs with severe hip OA and a control group of 5 sound dogs were used for this study. Results were statistically analyzed to detect a significant increase in peak vertical force (PVF) and vertical impulse (VI) in treated dogs. Mean values of PVF and VI were significantly improved within the first three months post-treatment in the OA group, increasing 9% and 2.5% body weight, respectively, at day 30. After this, the effect seems to decrease reaching initial values.ConclusionIntraarticular ADMSC therapy objectively improved limb function in dogs with hip OA. The duration of maximal effect was less than 3 months.

Highlights

Regenerative medicine using Mesenchymal Stem Cells (MSC) alone or combined with Plasma Rich in Growth Factors (PRGF) is a rapidly growing area of clinical research and is currently being used to treat osteoarthritis (OA)
Recent investigations have shown that growth factors contained in platelet-rich plasma (PRGF) act as vehicles that could act as potentiators or even extend the effect of MSCs [6,15,16]
If previous subjective studies seem to show certain effects, our research hypothesis should be that this effect could be detected and quantified by kinetik devices such as force platforms.The aim of this study was to use force platform kinetic analysis to demonstrate that the effect of a single intraarticular injection of Adipose-derived mesenchymal stem cells (ADMSCs) alone is more limited with respect to time, compared with ADMSCs + PRGF in 10 dogs with hip OA

Summary

Introduction

Regenerative medicine using Mesenchymal Stem Cells (MSC) alone or combined with Plasma Rich in Growth Factors (PRGF) is a rapidly growing area of clinical research and is currently being used to treat osteoarthritis (OA). Recent investigations have shown that growth factors contained in platelet-rich plasma (PRGF) act as vehicles that could act as potentiators or even extend the effect of MSCs [6,15,16]. This may be explained by the antiinflammatory effect of PRGF, which could help the MSCs differentiate in a more favorable environment [3]. The obtained results were compared with other medical strategies in canine OA and the advantages and disadvantages discussed

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