Assessment of the Effect of Complete Denture Adhesives on Oral Epithelial Cells’ DNA in Elderly Patients with Controlled Diabetes

Abstract

Aim of the study: Denture adhesives have long been recognized by denture wearers as a valuable assistant to denture retention and stability especially for patients reporting dissatisfaction with conventional dentures as diabetic patients. There is no available guide to select the proper adhesive form with least effect on oral mucosa. This clinical study assessed the impact of different complete denture adhesive forms on oral epithelial cells’ DNA in elderly patient with controlled diabetes.Materials and Methods: Fifteen completely edentulous controlled diabetic patients were selected and complete dentures were constructed. After two weeks of complete denture insertion, patients were prescribed with denture adhesives for another two weeks. According to the used adhesive form, patients were divided into three equal groups; group (I) used denture adhesive paste, group (II) used denture adhesive powder, where group (III) used denture adhesive cushion. The maxillary alveolar mucosae of all patients were swabbed to collect epithelial cells before denture insertion, two weeks after wearing the denture and two weeks after adhesive application. DNA analysis was carried out for the swabbed epithelial cells. The percentage of Genomic Template Stability (GTS %) was assessed and compared for all patients at various time intervals. Results: Genomic Template Stability decreased significantly in all patients two weeks after denture insertion while, significant increase in GTS% was observed two weeks after denture adhesives application. Cushion adhesive showed a significant increase in GTS% compared to paste and powder adhesives. Conclusion: Genomic Template Stability of controlled diabetic denture wearers improved when paste, powder or cushion adhesives were used. Cushion adhesive had a better effect on GTS% compared to paste or powder adhesives.