Assessment of the DNA barcoding for identification of Trigonostigma somphongsi, a critically endangered species in Thailand

Abstract

Trigonostigma somphongsi, a critically endangered species, is a rare and endemic fish in Thailand. This species had disappeared from its natural habitat for 20 years until 2006. The DNA barcodes or the fragments of cytochrome c oxidase I (COI) of T. somphongsi were investigated for species identification. The remaining two native species in the genus Trigonostigma, T. heteromorpha and T. espei were also identified using Boraras urophthalmoides as an outgroup species. The 707-bp fragments were successfully amplified and sequenced in all fifteen fish samples. In the genus Trigonostigma, the genetic distance within and between species ranged from 0.000 to 0.005 and 0.016 to 0.039, respectively. The lowest genetic distance (0.016) was between T. heteromorpha and T. espei, while the highest genetic distance (0.039) was between T. somphongsi and T. espei, followed by T. somphongsi and T. heteromorpha (0.035). The phylogenetic analysis showed that the relationship between the three Trigonostigma species (T. somphongsi was clearly separated from T. heteromorpha and T. espei) agreed with the morphological characteristics. These results suggest that DNA barcoding is an effective approach to identify Trigonostigma species for use in the conservation and management of fisheries.