Abstract

Assessment of the Diagnostic Performance of Normal and Irradiated Schistosoma mansoni Cercarial Antigens using ELISA

Highlights

  • MATERIALS AND METHODSSample collection Animal ethics approval was obtained from the deanship of scientific research, King Faisal University

  • 145 were evaluated for their depigmentary role and observed reciprocal expression against the TYR gene d expression

  • D o adapted to harsh desert environments (Eshra and c Badawy, 2014). It is raised for milk, meat and wool production and its demand is increasing globally n integrating into the international economy (Faye and U Bonnet, 2012; Faye, 2018). It is useful in agriculture, Tyrosinase is an enzyme that catalyzes the biosynthesis of melanin through various metabolic pathways producing quinone, which is the precursor for melanin pigmentation

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Summary

MATERIALS AND METHODS

Sample collection Animal ethics approval was obtained from the deanship of scientific research, King Faisal University. Dromedary camel samples (n=16) white (wodh) (4), black (majahim) (3), dark brown (sufor) (4) and diluted (shual) (5) including male and female were selected based on their phenotypic variation from the Camel Research Center at King Faisal University is presented in Supplementary Figure S1. Animals prepared aseptic with iodine, shaved the selected area and marked. Robbins Instruments Disposable Biopsy Punches (3 Mm) were used to collect the skin samples by rotating gently without applying pressure and cutting the sample with surgical scissors. Iodine and antiseptic powder was applied to the wound and it was monitored further to check for any infections. The collected samples were placed in sterile tubes with icebox and transferred to the lab and stored at -20°C until further processing

Universal primer AACTGGTGTCGTGGAG
DISCUSSION
Findings
CONCLUSION

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