Assessment of The Bioremediation Potential of Selected Bacterial Species Isolated from the Textile Printing Wastewater Inoculated with Cow Dung

Abstract

This study was conducted to isolate and identify bacteria from the wastewater of the textile printing industry inoculated with a mixture of cow dung, jaggery, and urea to assess the bioremediation potential of isolated bacterial species for reducing color and other polluting parameters of the wastewater. Based on colony characteristics, we were able to isolate nine types of bacteria (Pri 1 to 9), capable of thriving in textile printing wastewater. Out of the nine isolates examined, four (Pri 3, Pri 4, Pri 8, and Pri 9) significantly reduced the color and values of other physicochemical parameters of the wastewater. Isolated bacterial cultures were identified using various biochemical tests, antibiotic sensitivity tests, and rRNA sequence analysis. At the end of a 24-h incubation period at room temperature under shaking conditions at 100 rpm on an orbital shaker, isolate Pri 3 was identified as Alcaligenes aquatilis LMG 22996 (T), capable of reducing color by 86.13%, biological oxygen demand (BOD) by 70.44%, chemical oxygen demand (COD) by 80.65%, total dissolved solids (TDS) by 47.31%, total suspended solids (TSS) by 56.56%, and ammoniacal nitrogen by 75.95%; isolate Pri 4 as Priestia aryabhattai B8W22(T), capable of reducing color by 78.35%, BOD by 66.35%, COD by 74.92%, TDS by 34.94%, TSS by 31.66%, and ammoniacal nitrogen by 71.14%; isolate Pri 8 as Citrobacter werkmanii NBRC 105721(T), capable of reducing color by 90.37%, BOD by 82.13%, COD by 85.06%, TDS by 54.83%, TSS by 61.97%, and ammoniacal nitrogen by 80.76%; and isolate Pri 9 as Shewanella chilikensis JC5(T), capable of reducing color by 90.17%, BOD by 84.68%, COD by 83.46%, TDS by 59.13%, TSS by 62.45%, and ammoniacal nitrogen by 90.37%.