Assessment of the Biochemical Parameters of the Second Filial Generation Pups From the Wistar Rats Fed with Calcium Carbide Ripened Orange

Abstract

Fruits are useful food substances that provide unique and essential nutrients for the body. This present study is aimed at evaluation the toxic effect on the Biochemical indices of the second filial generation pup from the Wistar rats fed with Calcium Carbide forced ripened orange fruits. Mature unripe oranges were plucked off from the orange plant in Ogu, Yenagoa, Bayelsa state. They were forced to ripe with 10g calcium carbide which was placed in a bowl containing 5ml of water apparently used to dissolved it in a closed metal bucket containing 1kg of the matured orange rapped with black nylon and was kept two days [48 hours] to ripe. After ripening, sampled fruits were washed and juiced. 600g of calcium carbide forced ripened orange was peeled and blended in an electric blender with 350ml/L of distilled water to form the orange juice. The orange juice was filtered with a clean fine sieve and was poured into clean bottles labeled [CaC2 forced ripened orange juice] which was then stored in a refrigerator for further usage. 21 adult Wister rats [10 males and 11 females] weighing between 126.9- 213.3g were used for this study. The experimental Wistar rats were cohort into two and was allowed to acclimatize for two weeks (fed with grower mash with clean water ) at libitum then, different dosage of the fruit juice were administered orally based on their body weight. Group 1: Normal control group of 6 rats [3 males and 3 females] receive normal water and feeds only as placebo. Group 2: Treatment Group [2] of 6 rats [3 males and 3 females] received Calcium Carbide ripened orange juice. The treatment lasted for four weeks. The adult Wistar rats were allowed to copulate freely during and after the acclimatization and treatment period. Wistar rats birthed and the Pups of the different groups were collected according to the treatment protocols. The parent Wistar rats was still kept in their distinctive cages without treatment but was allowed free access to feed on the growers mash, clean water and copulation at libitum. They birthed again for the second time [Second Filial Generation] and the Pups were weighed at birth, at one week and two weeks, then they were sacrificed at the end of the second week and blood samples were collected from the two distinctive cohorts for Biochemical assay. Biochemical assay was done and results indicates reduction all the tested indices; AST, ALT, ALP, Creatinine, Urea, Albumin, Tottal protein, Total Cholesterol, Bilirubin, Lactate Dehydrogenase[LDH] in the Second Filial Generation Pups of the Calcium Carbide treated Wistar rat. Nutrients from the fruit induced with Calcium Carbide consumed during pregnancy permanently impact on the developing fetus of the Wistar rats which is expressed later in life.