Assessment of the antidiabetic potential of Gymnemic acid as α-amylase and α-Glucosidase inhibitor using invitro and insilico tools

Abstract

Diabetes Mellitus is a metabolic disorder characterized by elevated blood glucose level caused due to deficiency of insulin secretion or insulin action. The management of the blood glucose level is an important approach in the control of diabetes mellitus complications. There are many divergent therapeutic strategies in the management of Type II diabetes. The inhibition of carbohydrate hydrolyzing enzymes such as α-amylase and α-glucosidase can be used to lower postprandial blood glucose levels. The objective of the present study is to evaluate the inhibitory potential of Gymnemic acid in various concentrations on α-amylase and α-glucosidase activities. Gymnemic acid and voglibose show the IC50 value of 68.12 and 46.23 on alpha amylase and IC50 value of 65.77 and 36.67 on alpha glucosidase activity respectively. Insilico docking study between α-amylase and α-glucosidase with gymnemic acid and voglibose depict the similar amino acid binding sites that substantiate that gymnemic acid might be used as a natural and cost-effective alternative to the synthetic drug. This study throws light on the anti-diabetic action of phytomedicine, Gymnemic acid, a component of Gymnema sylvestre.