Abstract
Several plant-derived antibody (plantibody) purification approaches have been assessed demonstrating high purification capacities, but low recoveries (<30%) and poor sorbent stability. Thus, application of sorbents with high recovery, low ligand leakage and stable under harsh sanitization conditions could improve the efficiency antibody purification from this source. This paper reports for the first time MAbsorbents assessments in plantibody purification from tobacco plants. In conclusion MAbsorbent A1P is not suitable for plantibody HB-01 purification, while MAbsorbent A2P demonstrated high recovery (37.54 ± 15.15%) in 25 purification cycles. As recommendation, we consider that other elution conditions should be assessed before restudy the stability of this sorbent.
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