Abstract
Red blood cells are still the most widely transfused blood component worldwide and their story is intimately entwined with the history of transfusion medicine and the changes in the collection and storage of blood.1,2 At present, the most widely used protocol for the storage of red blood cells (for up to 42 days) is the collection of blood into anticoagulant solutions (typically citrate-dextrose-phosphate); red cell concentrates are prepared by the removal of plasma and, in some cases, also leukoreduction. The product is stored at 4 ± 2° C in a slightly hypertonic additive solution, generally SAGM (sodium, adenine, glucose, mannitol, 376 mOsm/L).1 The British obstetrician, Braxton Hicks in 1868, experimented with a solution of phosphate of soda, but this also proved toxic. Richard Lewinsohn, in 1915, of the Mount Sinai Hospital in New York is credited with introducing sodium citrate into clinical practice as an anticoagulant.3In fact, a 1% solution of sodium citrate was already widely used in laboratories as an anticoagulant. This high concentration was toxic to humans but, as Lewinsohn himself recalled, `Nobody had ever followed the simple thought of carrying out experiments to ascertain whether a much smaller dose might not be sufficient' for use as an anticoagulant.
Highlights
Red blood cells are still the most widely transfused blood component worldwide and their story is intimately entwined with the history of transfusion medicine and the changes in the collection and storage of blood.[1,2] At present, the most widely used protocol for the storage of red blood cells is the collection of blood into anticoagulant solutions; red cell concentrates are prepared by the removal of plasma and, in some cases, leukoreduction
It is known that when red cell units are ex vivo preservation of blood both within the red cells stored in the blood bank due to inherent red cell storage and in the storage medium, these have been collectively lesions, changes occur both in red cells and the termed as red cell storage lesions
Though there is a spectrum of changes which takes place in red cell units during a) The most salient biochemical changes occurring in storage, the changes of clinical significance include red stored red cell units cell hemolysis and increased plasma potassium levels
Summary
Electrolytes (Na+, K+, Cl-) pH, LDH and Hemogram on day 0,1, 7, 14, 21, 28 in stored blood units. How are we to answer the question of blood donation camps These bags were examined for “What are the established and theoretical factors to various parameters during 28 days of storage period. Consider in assessing the red cell storage lesion?” As The study was conducted from details about the blood detailed here and as reviewed extensively elsewhere, donation and processing were recorded in the there is a well-defined list of factors that are traditional proforma. Blood Collection- Blood collection was done strictly it is unclear that 24-hour recoveries predict efficacy as per the guidelines prescribed by Directorate beyond eliminating lack thereof due to nonviable RBCs. Because some metrics can predict blood that has lost departmental protocols for donor selection and its integrity, in vitro measures remain a guiding factor in phlebotomy were followed.
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