Abstract
An experiment was conducted to characterize the changes in sperm membrane integrity (SMI;%) and sperm motility (MOT;%) in extended stallion semen following cooled storage for up to 10 days. Three gel-free ejaculates from each of five stallions (n1⁄415) were diluted in INRA 96 extender (IMV Technologies, L’Aigle, France) to a concentration of 50 x 106 sperm/mL, and then subjected to cushioned centrifugation at 400 x g for 20 min in glass nipplebottom tubes. Following removal of supernatant, sperm pellets were resuspended to approximately 30 x 106 sperm/ mL in the same extender containing 10% (v/v) fresh, homologous seminal plasma derived by centrifugation of raw semen and filtration of resulting seminal plasma from the same ejaculate. Ten aliquots of extended semen per ejaculate were stored in an Equitainer II (Hamilton Research, Inc., South Hamilton, MA, USA) for 24 h, then transferred to a refrigerator set at 4 C for up to 9 additional days. Sperm membrane integrity was evaluated daily by flow cytometry (FACScan; Becton Dickinson, Mountain View, CA) using three different fluorescent staining protocols: Pisum sativum agglutinin and propidium iodide (PSA-PI); SYBR-14, propidium iodide, and JC-1 (SYPIJC); and SNARF-1, Yo-Pro1, and ethidium homodimer (SYE). Dimethylsulfoxide was required as a solvent for some fluorescent probes, but final concentration of dimethylsulfoxide was held at less than
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
