Assessment of semen characteristics and in vivo conception rate of preserved buffalo bull semen extended in tris enhanced with Diospyros kaki

Reda Ibrahim El-Sheshtawy,Walid Said El-Nattat

doi:10.1186/s42269-020-00353-5

Reda Ibrahim El-Sheshtawy, Walid Said El-Nattat

Open Access

https://doi.org/10.1186/s42269-020-00353-5

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Abstract

BackgroundThe freeze-thaw process results in structural and functional damages caused by over accumulation of reactive oxygen species (ROS). Addition of exogenous antioxidants to semen extender is of a great importance to overcome the oxidative damage during the freezing process. The present study aimed to clarify the impact of Diospyros kaki on preserved buffalo semen quality. Tris extender enhanced with kaki juice (KEE) at concentrations 0% (control) and 1-10% (v/v). A twenty percent of freshly prepared egg yolk was added to the KEE extender (KEEY), then all tubes were centrifuged to eliminate any debris. Semen was added to the supernatants in other tubes. Semen was evaluated and the conception rate was implemented.ResultsSperm forward motility was significantly (P < 0.0001) kept high after 5 days of chilling for the concentrations 3, 4, and 5% with respect to the control and kept high (P < 0.01) at the other concentrations till the 4th day of chilling. Addition of KEE had significantly (P < 0.03) ameliorated post freezing sperm motility with all the concentrations of the extender except the concentration (10%). The highest spermatozoal motility was obtained with the concentration of 1% with respect to the control. Alive sperm%, abnormalities%, and % of intact spermatozoa membranes (HOST%) were similar to the control with all concentrations of kaki used. The conception rate was higher when 1-6% KEE were used.ConclusionsSome concentrations of Diospyros kaki improved buffalo bull semen quality post-chilling, post-freezing, the conception rate, and the concentration 1% gave the best results.

Highlights

The freeze-thaw process results in structural and functional damages caused by over accumulation of reactive oxygen species (ROS)
The aim of the study is to clarify the impact of Diospyros kaki of preserved buffalo semen quality
Kaki-enhanced extender (KEE) was set by supplementing variable concentrations 0.0 ml/5.0 ml, 0.5 ml/4.5 ml (1%), 1 ml/4 ml (2%), 1.5 ml/3.5 ml (3%), 2.0 ml/3.0 ml (4%), 2.5 ml/2.5 ml (5%), 3.0 ml/2.0 ml (6%), 3.5 ml/1.5 ml (7%), 4.0 ml/1.0 ml (8%), 4.5 ml/0.5 ml (9%), and 5.0 ml/0.0 ml (10%) to TCF to obtain a final volume of 5 ml

Summary

Introduction

The freeze-thaw process results in structural and functional damages caused by over accumulation of reactive oxygen species (ROS). Persimmon (Diospyros kaki) fruit was one of those plants having high nutritive value and inclosed many biologically active substances including antioxidants, triterpenoids, fiber, and minerals (Gorinstein et al, 2001; Chen et al 2008; Park et al 2008; Akter and Eun 2009; Veberic et al 2010; Zhou et al 2010; Dembitsky et al 2011). It had multiple medicinal values, according to the variety of antioxidants involving phenolics, carotenoids, and vitamin C (George and Redpath 2008). The aim of the study is to clarify the impact of Diospyros kaki of preserved buffalo semen quality

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