Abstract
Introduction: Semen is considered a diagnostic fluid in forensic science which helps in diagnosis of sexual assault. The present study was conducted to determine semen biomarkers in vaginal fluid in suspected rate victims. Materials & Method: The present study found 20 rape victim out of 94 women. In all, vaginal swabs were obtained. In this, Semen cytology (SC), prostate-specific antigen (PSA) and Y chromosome DNA was detected with polymerase chain reaction. Results: Maximum victims were seen in age group 10-20 years (32) followed by 20-30 years (29), 30-40 years (28), 40-50 years (4) and >50 years (1). It was found that 20 cases were found to be positive of rape. Sensitivity of SC was found as 70%, PAS as 80% and PCR as 100%. Specificity of SC was 88%, PSA 92% and PCR as 96%. Table III shows various primers used in the study. Conclusion: Semen biomarkers such as SC, PSA and Yc-DNA are widely used and accepted methods for detection of offense. They have high specificity and sensitivity rate.
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