Abstract
Blood samples from 255 child bearing age women distributed into two villages named EL Nuba and EL Massoudia were collected according to their agreement and after obtaining their written consent form. Plasma separated from 255 blood samples were tested at delivery for Toxoplasma gondii antibodies using three different screening tests. These tests were Latex Agglutination Test (LAT), Complement Fixation Test (CFT), and ELISA IgG. Evaluation of the screening test was done for the three tests. The sensitivity of LAT, CFT, and ELISA IgG were 82.5%, 96.8%, and 98.9% respectively. The specificity of these tests was 91.2%, 94.1%, and 97.1% respectively. The Positive predictive value was 96.2%, 97.8%, and 98.9% respectively. The negative predictive value was 66%, 91.4% and 97.8%, respectively. The positive likelihood ratio was 9.35, 16.4, and 33.6 respectively. The negative likelihood ratio was 0.192, 0.0345, and 0.11 respectively. According to these evaluations a proposed protocol was drawn to detect infection in women.
Highlights
Diagnosis of toxoplasmosis in humans is performed using different techniques
In this study three different screening tests were used to detect the Toxoplasma gondii antibodies in childbearing age women
Screening tests were evaluated through calculation of the sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, positive predictive value, negative predictive value, and odds ratio
Summary
Diagnosis of toxoplasmosis in humans is performed using different techniques. A few examples of these techniques are mouse inoculation, detection of anti-Toxoplasma antibodies, histological demonstration of tachyzoites in tissue sections or smears of body fluid, and detection of Toxoplasma gondii DNA by molecular methods [1,2,3].Many serological methods for the diagnosis of toxoplasmosis have been established over the years. Diagnosis of toxoplasmosis in humans is performed using different techniques. A few examples of these techniques are mouse inoculation, detection of anti-Toxoplasma antibodies, histological demonstration of tachyzoites in tissue sections or smears of body fluid, and detection of Toxoplasma gondii DNA by molecular methods [1,2,3]. Many serological methods for the diagnosis of toxoplasmosis have been established over the years. Most have been developed for detection of Toxoplasma infection in human and commercially available in kit form [4]. Detection of anti-Toxoplasma antibodies indicates that a person has been infected with Toxoplasma gondii some time in the past. There are many different serological techniques available, e.g. the dye test, the complement fixation test, the indirect immunofluorescent test, the latex agglutination test, the enzyme linked immunosorbent assay, and the immunosorbent agglutination test [5,1]
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