Abstract
Boar semen is voluminous and ejaculated as jets or fractions of pre-sperm, sperm rich (SRF) and post-sperm rich fractions. Recent studies have reported more resilient characteristics of sperm in initial portions of SRF towards cold shock and cryopreservation. The present study was conducted to assess the quality of specific fractions of SRF, namely, first 10mL of SRF (F1) and rest of SRF (F2) in Large white Yorkshire (LWY) boar semen. Ejaculates were collected using gloved-hand technique and were subjected to quality assessments of volume, pH, sperm progressive motility, concentration, plasma membrane integrity, abnormality, acrosome integrity and sperm membrane cholesterol. Upon statistical analysis, significant differences were noticed in volume, pH, sperm concentration and sperm membrane cholesterol between fractions of the ejaculate.
Highlights
Boar semen is voluminous and ejaculated as jets or fractions of pre-sperm, sperm rich (SRF) and post-sperm rich fractions
Recent studies have reported that the sperm fortuitously in first 10mL of SRF (F1) were resilient to cooling, freezing and thawing because of its lower bicarbonate level and presence or absence of specific proteins
A total of 66 semen ejaculates were collected using gloved hand technique at twice a week frequency with an interval of three to four days between collections from the same boar.Ejaculates were collected as fractions, with the first 10 mL of SRF being designated as F1 and the remaining SRF as F2
Summary
Boar semen is voluminous and ejaculated as jets or fractions of pre-sperm, sperm rich (SRF) and post-sperm rich fractions. Assistant Professor, Centre for Pig Production and Research, KVASU, Mannuthy 6. Boar semen is voluminous and ejaculated as jets in broadly three fractions as (i) pre-sperm (ii) the SRF and, (iii) the post-sperm-rich (PSRF) which differ in sperm concentration and proportion of accessory sex gland secretions (Mann and Luwak-Mann, 1981).
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