Abstract
Pru p 1 and Pru p 3 are proteins found in peach (Prunus persica (L.) Batsch), and they are members of the pathogenesis-related protein 10 (PR-10) and non-specific lipid transfer protein (nsLTP) families, respectively. They are the main allergens of peach. In this paper, we describe a quantitative method for these proteins based on protein absolute quantification with liquid chromatography–tandem mass spectrometry, and LVASPSGGSIIK[13C6,15N2] and ISASTNC[CAM]ATVK[13C6,15N2] (C[CAM]: carbamidomethyl-modified C), stable isotope-labelled internal standard peptides. We show that this method exhibits linearity with r2 > 0.97 in a dose range of 3.1–200 fmol/μL for LVASPSGGSIIK[13C6,15N2] and 13–200 fmol/μL for ISASTNC[CAM]ATVK[13C6,15N2]), and overall coefficients of variation for multiple tests of 11.6–30.1%. This approach is therefore helpful for screening and breeding low allergenic peach cultivars.
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