Assessment of Protein Content and Study Effect of Anti-Nutrition Factor on in-vitro Protein Digestibility

Abstract

Aims of this study were assessed the protein content of dry meat, raw egg, dehulled seeds of wheat, pigeon pea and study effect of concentration 1, 2 and 3% of the Pure Tannin, Solenostemma argel, Artemisia herba alba , Process green tea and Moringa oleifera on in-vitro protein digestability of animal and plant protein. Experiment: It was carried out in lab of Department of Food Science and Technology, Faculty of Agriculture, University of Khartoum in 2014. Methodology: Micro-Kjeldhal method and in-vitro protein digestability were used. Results: Results indicated that protein content of dry meat (77%) and raw egg (79%) was higher than protein content of dehulled seeds of wheat (18%) and dehulled seeds of pigeon pea (22%). There was significant difference between the four samples in protein at P ≤ 0.05. In-vitro protein digestability of dry meat, raw egg, dehulled seeds of wheat and pigeon was clearly reduced with increasing the concentration of pure tannin. While in-vitro protein digestability for dehulled wheat was clearly increased with increasing the concentration of S . argel leaves, but no significant was observed in dehulled seeds of pigeon pea, but at concentration 3% S. argel, the in-vitro protein digestability was decreased. In case of dry meat and raw egg in-vitro protein digestability was reduced with adding 1, 2 and 3% S. argel leaves. Whereas, in-vitro protein digestability for dehulled seeds of wheat was improved with increasing concentration of S. argel , but in-vitro protein of dehulled pigeon pea was decreased with increasing concentration of S. argel . While in-vitro protein digestability for dry meat, raw egg, dehulled seeds of wheat and pigeon pea was reduced with increasing concentration of A. herba alba , process green tea and M. oleifera , but at concentration 3% of moringa the in-vitro protein digestability of dehulled seeds of pigeon pea (75%) was improved. Conclusion: Protein content is very rich in animal products, but it is very poor in plant products. Uses of S. argel with dehulled seeds of wheat was improved its in-vitro protein digestability, but uses of pure tannic acid, A. herba alba and green tea reduced in-vitro protein digestability.